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Sequence tag preprocessing was performed according to a 1116 described protocol with some modifications. To investigate the expression profile of BnaC9. Therefore, the deletion of BnaC9. S14B ; Supplemental Table S The lengths of the branches refer to the amino acid variation rates.

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This pattern of apical infection and release of virus in polarized epithelia is reminiscent of transmissible porcine gastroenteritis virus 36 and HCoVE The results indicated that relatively low expression of BnaC9.

Based on these 111416, we proposed a model to describe the possible evolutionary process from AtSMG7 to its homologs in B.

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The planting density was 20 cm between plants within rows and 25 cm between rows. Lung pathology of fatal severe acute respiratory syndrome. C9we field planted a BC 4 F 2 population consisting of 17, individuals. As expected, we found that normal FGs appeared in most of the ovules As en by immunofluorescence staining and membrane biotinylation, ACE2 protein was more abundantly expressed on the apical than the basolateral surface of polarized airway epithelia.

Number of seeds per silique NSS is an important determinant of seed yield potential in Brassicaceae crops, and it is controlled by naturally occurring quantitative trait loci. Support Center Support Center. Consistent with the qPCR analysis, GUS activity was gradually enhanced in the pistils before anthesis and decreased after anthesis. To validate a model to test this hypothesis, we used SEM to compare the apical surface morphology of well-differentiated epithelia with that of well-differentiated cells subsequently grown with medium present on their apical surfaces for 7 days.

The apical expression of ACE2 on epithelia indicates that this coronavirus receptor is accessible for topical application of receptor antagonists or inhibitors. SMG7b are still retained among modern B. Therefore, the presence of BnaC9.

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Supplementary Material Supplemental Data: The effects of coronavirus on human nasal ciliated respiratory epithelium. Thus, it is highly desirable to identify the genes responsible for each component and subsequently, understand the genetic mechanisms underlying the natural variations. The results showed that, except for eight accessions that did not harbor BnaC9. As a complex quantitative trait, the seed yield of a Lwi.

SMG7b in the B. SMG7b itself or a tightly linked neighboring member. MHV-A59 enters polarized murine epithelial cells through the apical surface but is released basolaterally. Black lines represent introns. National Center for Biotechnology InformationU.

Open in a separate window. SMG7call other B. SMG7b and nine accessions that did not harbor BnaC9. Please review our privacy policy. A, Fine mapping of the qSS. Arabidopsis Arabidopsis thaliana plants and transgene-positive and -negative B.