AKTA PURIFIER MANUAL PDF

About this manual. This manual describes the operation of the ÄKTA™FPLC™ system. System description, system maintenance and trouble-shooting are also. filter. Sample pump. Buffer select valve (V6). Injection valve (V1). Reverse . Manual. Flowpath. B1 or B2. BufferValveB. Pump A. Pump B. Common inlet. A2. A1. About this manual This manual describes the operation of ÄKTA purifier: Evaluating the UV response Calculate the UV response ratios in the following.

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If the system will be used with other buffers the next day, rinse the pump and the system with distilled water using the PumpWash instruction as follows: Click on the Y-axis and Xaxis tabs to set the scale for the different curves. Zwitter ions Using zwitter ions in a BufferPrep recipe can be difficult.

Never place the reservoir flask below the level puifier the pump inlet. Note that the term chromatogram is used here when talking about the whole window containing all the different curves. Cables, capillaries, accessories, column holder, etc. Remove all red tape holding capillaries and loops attached to valve INV Put the open end in a waste container.

Let the system dry completely before using it.

The fractionation order can be selected as serpentine-row, row-by-row, serpentine-column or column-by-column. Close the Documentation window by clicking the X in the upper right corner. If there are signs of liquid leaking between the pump head and the housing side panel or increased or decreased volume of rinsing solution, replace the piston seals. For low-pressure columns, at high flow rates. Material Color Volume of 10 cm A1, B1 cm 1.

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For continued protection against risk of fire, replace only with a fuse of the specified type and rating. Consider the maximum allowed pressure for the column and the size of the column.

ÄKTApurifier – GE Healthcare Life Sciences

For more information on: The Declaration of conformity is valid only for systems that are marked with the CE logo:: If any of the evaluated values falls outside the specified range, go to Section 2.

If air is allowed to enter the columns, their performance can be adversely affected or destroyed. Before moving the system, disconnect all cables and tubing connected to peripheral components and liquid containers. In a domestic environment, it may cause radio interference, in which case the user may be required to take suitable measures. The run will start. If the pH and conductivity curves indicate uneven mixing of your buffers unstable curveschange to manuwl larger mixer chamber. It is divided into two parts, one describing wkta installation and one describing how to run the installation test.

The pH electrode is delivered with a transparent cover. If there is a risk that large volumes of spilt liquid may penetrate the casing of the instruments and come into contact with the electrical components, immediately disconnect the mains cable and contact an authorized service technician. The illustration to the right shows setting of the wavelength nm. Click OK at the bottom of the Chromatogram Layout window.

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ÄKTApurifier

The Page Setup dialog opens and you can, for example select page size and items to be included in the header and in the footer. Chemical stains and dust should be removed. Superloop 10 ml and Superloop 50 ml must not be used at pressures above 4 MPa 40 ppurifier, psi.

Filling Superloop Filling Superloop is achieved as follows: Do not tighten TeflonTM tubing further as this will damage the end of the tubing.

Manual menu select Other. If tubing with too large inner diameter is used, the peaks will become broader purifire necessary. The name is followed by a three-digit sequence number starting with Columns are snapped in place in column holders. The system name is displayed in the title bar of the window. The method is held in its current status.

Then, when running the method, use a piece of tubing to replace the purified. When the peak end is detected, the fixed volume fractionation continues. For analytical reproducibility, use a sample volume that is 5 times the volume of the sample loop. Do not disassemble the optical unit while the lamp is ON.